CRISPR-TAPE

CRISPR-TAPE provides protein-centric gRNA design for CRISPR-based protein engineering, enabling targeting of specific amino acid residues or residue types to facilitate precise proteome modifications and homology-directed repair (HDR).

Key Features:

• Protein-Centric Design: Implements a protein-centric gRNA design algorithm that focuses on individual amino acid residues and residue types rather than gene-centric loci.
• Customizable gRNA Outputs: Generates customizable gRNA outputs with options tailored to maximize the efficacy of homology-directed repair (HDR).
• Automated gRNA Library Generation: Automates the gRNA design process and reduces manual curation of gRNA libraries for targeted proteome engineering.
• Reduced Computational Load: Optimizes processing to decrease CPU times for large-scale analyses.

Scientific Applications:

• Targeted Proteome Engineering: Enables residue-level targeting for precise modifications of protein sequence and function.
• HDR-focused Genome Editing: Supports design of gRNAs intended to improve homology-directed repair outcomes for precise genetic modifications.
• Genomics and Proteomics Research: Facilitates studies requiring specific protein alterations and scalable analyses due to optimized computational performance.

Methodology:

Uses a protein-centric gRNA design algorithm that automates gRNA library generation, produces customizable outputs optimized for homology-directed repair (HDR), and is optimized to decrease CPU times.