CRISPR-GRANT

CRISPR-GRANT performs high-throughput indel (insertion-deletion) analysis of genome editing events induced by the CRISPR/Cas system from next-generation sequencing (NGS) data.

Key Features:

• Indel analysis: Performs indel (insertion-deletion) detection and quantification of CRISPR/Cas-induced genome edits.
• High-throughput evaluation: Processes large NGS datasets to support high-throughput assessment of editing events.
• Sample-type support: Conducts one-step analysis for single amplicons, pooled amplicons, and whole-genome sequencing (WGS) datasets without separate pre-processing.
• Computational efficiency: Is optimized for shorter run-times compared to existing indel analysis tools.

Scientific Applications:

• Functional genomics: Enables quantification of CRISPR/Cas-induced indels to assess gene function and mutational outcomes.
• Genetic engineering: Supports evaluation of editing efficiency and indel spectra for genome engineering experiments.
• Translational biomedical research: Facilitates analysis of DNA editing events in studies aiming to translate CRISPR/Cas interventions to biomedical contexts.

Methodology:

Performs one-step computational analysis of NGS data without separate pre-processing, handling single and pooled amplicons as well as whole-genome sequencing (WGS) datasets, and employs optimizations to reduce run-time compared with existing indel analysis tools.