CRISPRware

CRISPRware generates guide RNA (gRNA) libraries against transcribed, translated, and noncoding regions by leveraging next-generation sequencing data to enable context-specific and allele-specific guide design on a genome-wide scale.

Key Features:

• Context-specific gRNA design: CRISPRware leverages next-generation sequencing data to design gRNAs tailored to specific genomic contexts.
• Allele-specific guide design: CRISPRware accounts for genetic variation to enable allele-specific guide design on a genome-wide scale.
• Multi-target coverage: CRISPRware designs gRNAs against transcribed, translated, and noncoding regions.
• Multi-organism support: CRISPRware identifies and scores gRNAs against coding sequences in six model organisms for Cas9 and Cas12A.

Scientific Applications:

• Genome-wide gRNA library generation: CRISPRware generates comprehensive gRNA libraries for CRISPR-based functional genomics screens.
• Allele-specific gene editing: CRISPRware enables precise targeting of specific alleles by accounting for genetic variation in guide design.
• Cas9 and Cas12A guide design: CRISPRware supports gRNA design for both Cas9 and Cas12A nucleases across multiple model organisms.

Methodology:

CRISPRware leverages next-generation sequencing data and accounts for genetic variation to design and score gRNAs.