CrossWork

CrossWork analyzes chemical cross-linking tandem mass spectrometry data to identify cross-linked peptides and derive structural distance constraints for protein structure and interaction analysis.

Key Features:

• Standard Cross-Linkers: Supports analysis of data generated with BS3 (bis(sulfosuccinimidyl)suberate) and BS2G (bis[sulfosuccinimidyl]glutarate).
• Efficient Data Processing: Processes batches of tandem mass-spectrometric data and distinguishes cross-linked from non-cross-linked peptides.
• High Sensitivity and Accuracy: Validated on small-scale (single protein) and large-scale (thousands of proteins) search spaces, producing distance constraints that align with established protein structures.

Scientific Applications:

• Protein Structure Analysis: Generates cross-link-derived distance constraints to inform structural models of large and complex proteins.
• Interaction Mapping: Identifies cross-linked peptides to map protein-protein interactions.
• Multi-Domain Protein Studies: Integrates cross-link data to study multi-domain proteins such as ERp72 and to combine individual domain structures into cohesive models.

Methodology:

Performs chemical cross-linking with BS3 or BS2G, analyzes resulting peptides by tandem mass spectrometry, and computationally identifies cross-linked peptide pairs to derive structural constraints.