cshl_princeton_fastx_barcode_splitter

cshl_princeton_fastx_barcode_splitter splits FASTQ and FASTA sequencing reads by barcode to organize multiplexed NGS data for analysis of DNA- and RNA-based experiments.

Key Features:

• Barcode-Based Splitting: Uses barcodes embedded within sequencing reads to segregate reads into distinct output files.
• Support for FASTQ/FASTA Formats: Accepts FASTQ and FASTA input formats for nucleotide sequencing reads.

Scientific Applications:

• NGS Data Analysis: Enables separation of sequencing reads by barcode to support NGS studies, including analysis of bacterial RNA polymerase promoter melting activity across numerous DNA sequence variants.
• Facilitation of In Vitro Experiments: Allows parallel handling of thousands of sequences to accumulate data on sequence-dependent behavior of proteins acting on DNA.

Methodology:

Leverages barcodes as the criterion for splitting sequencing reads into separate files; automates data organization to support downstream analyses.