CTFMeasure

CTFMeasure estimates contrast transfer function (CTF) parameters and absolute tilt angle offset in cryo-electron tomography tilt series.

Key Features:

• Tilt-series-based joint estimation: CTFMeasure estimates CTF parameters together with the absolute tilt angle offset of the sample.
• Thon ring rescaling: The tool applies Thon ring rescaling to improve estimation accuracy.
• FIB milling lamella utilities: CTFMeasure provides utilities for focused ion beam (FIB) milling lamellas.
• Power spectra visualization: The tool supports visualization of power spectra for analysis.

Scientific Applications:

• Cryo-electron tomography data processing: CTFMeasure enables CTF estimation essential to the cryoET data processing workflow.
• Tilt series analysis: The tool estimates CTF parameters and absolute tilt angle offset of input tilt series for structural studies.

Methodology:

CTFMeasure utilizes tilt-series-based joint estimation and Thon ring rescaling.