CYANUS

CYANUS performs differential expression analysis of cytometry data at single-cell resolution to identify differences in cellular marker expression across conditions.

Key Features:

• Differential Expression Analysis: Identifies differences in marker expression between conditions in cytometry data without relying on simplified distributional assumptions.
• CyEMD (Cytometry Earth Mover’s Distance): Calculates the Earth Mover’s Distance between expression distributions to handle strong zero-inflation while reducing sensitivity to noise and overprediction.
• Integrated, Evaluated Methods: Integrates well-performing existing and novel methods identified through systematic evaluation on real and simulated CyTOF (Cytometry by Time-Of-Flight) data.

Scientific Applications:

• Immunology: Enables marker-level differential expression and discovery of cellular subpopulations relevant to immune phenotyping.
• Oncology: Supports identification of subtle cellular characteristics and marker changes associated with tumor biology and therapeutic response.
• Developmental Biology: Facilitates detection of differential marker expression across developmental states at single-cell resolution.

Methodology:

Computes Earth Mover’s Distance between expression distributions via CyEMD and performs systematic evaluation of existing and novel methods on real and simulated CyTOF data to address zero-inflation.