CysModDB

CysModDB curates and provides annotated, experimentally validated cysteine posttranslational modification (CysPTM) sites and associated protein annotations to support analysis of CysPTM roles in protein function and cellular regulation.

Key Features:

• Experimental site collection: Contains over 70,536 experimentally validated CysPTM sites compiled from curated datasets.
• Protein annotations: Provides annotations for 21,654 modified proteins including functional regions and structural information.
• Sample and experimental metadata: Annotates each CysPTM site with sample origin and the enrichment techniques used for identification.
• Structural and region mapping: Maps CysPTM sites onto protein structures and defined functional regions to contextualize modification locations.
• Cross-references: Links CysPTM sites and proteins to external databases, including protein–protein interaction resources.
• Predictive tools: Offers computational tools for predicting potential CysPTM sites from sequence or protein context.
• Integrated analyses: Includes tools for gene enrichment analysis and investigation of sequence features associated with CysPTMs.
• Manual curation: Integrates manually curated resources to ensure experimental verification and annotation consistency.

Scientific Applications:

• Functional regulation studies: Enables investigation of how CysPTMs influence protein function and structural properties.
• Signaling and network analysis: Supports analysis of CysPTM roles within protein–protein interaction networks and signaling pathways.
• Disease mechanism research: Facilitates study of CysPTM involvement in disease mechanisms and identification of potential therapeutic targets.
• Hypothesis generation: Uses predictive site models and integrated data to generate hypotheses for experimental validation.

Methodology:

Manually curated experimental CysPTM data were integrated and annotated with sample origin and enrichment techniques, mapped onto protein structures and functional regions, and cross-referenced to external databases.