CytoSeg

CytoSeg extracts actin filaments (AFs) from fluorescence microscopy images and represents them as networks for quantitative analysis of actin cytoskeleton organization and dynamics.

Key Features:

• Automated image-based framework: Automatically extracts AFs from fluorescence microscopy images for downstream analysis.
• Network representation: Represents extracted AFs as network graphs to enable structural and topological analyses.
• Feature identification and comparison: Performs automated analysis of network representations to identify and compare biologically relevant features of actin organization.
• Noise and dynamics handling: Processes images to address low signal-to-noise ratios and dynamic structural changes in actin filaments.

Scientific Applications:

• Cellular dynamics analysis: Quantifies actin network organization and dynamics in live-cell fluorescence microscopy studies.
• Cell motility and morphology: Relates changes in AF network structure to cell motility and morphology alterations.
• Comparative studies: Enables quantitative comparison of actin organization across different biological conditions or time points.

Methodology:

Processes fluorescence microscopy images to extract actin filaments, converts extracted filaments into network representations, and performs automated network-based feature identification and comparison while addressing low signal-to-noise ratios and dynamic structural changes.