dada2

DADA2 is an open-source software package for modeling and correcting errors in Illumina-sequenced amplicons. The tool accurately infers sample sequences and resolves differences as minor as a single nucleotide. Compared to other methods, DADA2 demonstrated superior performance in identifying actual variants while producing fewer spurious sequences, as evidenced in several mock communities.

Topic

Sequencing;Genetic variation;Microbial ecology;Metagenomics

Detail

  • Operation: Variant calling;DNA barcoding

  • Software interface: Command-line user interface,Library

  • Language: R

  • License: The GNU General Public License v3.0

  • Cost: Free

  • Version name: 1.30.0

  • Credit: NSF,NIH, the Samarth Foundation (Stanford Microbiome Seed Grant.

  • Input: -

  • Output: -

  • Contact: Benjamin Callahan benjamin.j.callahan@gmail.com

  • Collection: -

  • Maturity: Stable

Publications

  • DADA2: High-resolution sample inference from Illumina amplicon data.
  • Callahan BJ, et al. DADA2: High-resolution sample inference from Illumina amplicon data. DADA2: High-resolution sample inference from Illumina amplicon data. 2016; 13:581-3. doi: 10.1038/nmeth.3869
  • https://doi.org/10.1038/nmeth.3869
  • PMID: 27214047
  • PMC: PMC4927377

Download and documentation

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