Database for Bacterial Group 2 Introns

Database for Bacterial Group 2 Introns catalogs full-length, non-redundant group II introns from bacterial DNA sequences archived in GenBank and provides sequence, insertion-site, encoded-protein, and RNA secondary-structure data for their analysis.

Key Features:

• Comprehensive Catalogue: Full-length, non-redundant entries of bacterial group II introns compiled from GenBank sequences.
• General Information: Curated factual content on group II intron properties, RNA structures, and classification systems.
• Detailed Intron Data: Per-intron annotations including genomic insertion sites, intron DNA sequences, encoded protein sequences, and RNA secondary-structure models.
• Analytical Tools: Integrated local BLAST for finding closest sequence relatives and a boundary-finding tool to predict 5' and 3' intron–exon junctions.
• Data Export: Selected intron sequence data provided in FASTA format for downstream analysis.

Scientific Applications:

• Evolutionary analysis: Comparative sequence and structure data support studies of group II intron evolution and phylogenetics.
• Genome annotation: Sequence and junction-prediction information assists identification and characterization of group II introns in bacterial genomes.
• RNA and protein function studies: RNA secondary-structure models and encoded-protein sequences enable investigations into splicing mechanisms and coding potential.

Methodology:

Compilation from bacterial DNA sequences archived in GenBank; curation to full-length, non-redundant intron entries; sequence-similarity searches using a local BLAST; boundary prediction of 5' and 3' intron–exon junctions using a boundary-finding tool; inclusion of RNA secondary-structure models; provision of selected sequences in FASTA format.