NCBI database of sequence tagged sites

NCBI database of sequence tagged sites provides a curated collection of sequence tagged sites (STSs) defined by specific PCR primer pairs with associated genomic positions, sequence data, and gene associations for use in genomic mapping and sequencing projects.

Key Features:

• PCR Primer Pairs: Each STS entry is defined by specific PCR primer pairs enabling targeted amplification.
• Genomic Positioning: The database includes detailed genomic coordinates for each STS.
• Gene Associations: STSs are linked to associated genes to indicate potential functional or regulatory relationships.
• Sequence Information: Full sequence data for each STS is provided for analysis and comparison.

Scientific Applications:

• Genomic mapping: Provides anchors for constructing physical maps and locating loci within genomes.
• Sequencing projects: Anchors sequencing and sequencing of model organisms' genomes by supplying mapped STSs.
• PCR-based validation: Enables PCR identification and amplification of loci using the provided primer pairs.
• Data integration: Facilitates merging diverse mapping data from different laboratories to create consensus physical maps.
• Clone collection management: Supports management of extensive collections of cloned DNA segments by supplying STS identifiers and positions.

Methodology:

Stores and distributes STS sequences, PCR primer pairs, genomic coordinates, and gene association annotations.