Deconwolf

Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images for accurate quantification of crowded diffraction-limited fluorescence dots and improved chromosome tracing.

Key Features:

• High-performance deconvolution: Deconwolf performs deconvolution of widefield fluorescence microscopy images with efficient execution on laptop computers.
• Accurate quantification of crowded targets: Deconwolf enables accurate quantification of crowded diffraction-limited fluorescence dots in DNA and RNA fluorescence in situ hybridization images.
• Robust transcript detection: Deconwolf allows robust detection of individual transcripts in tissue sections imaged with x20 air objectives.
• Enhanced spatial transcriptomics: Deconwolf increases the number of transcripts identified more than threefold in in situ spatial transcriptomics images.
• Improved chromosome tracing: Deconwolf drastically improves chromosome tracing in images obtained by fluorescence in situ sequencing of barcoded Oligopaint probes.

Scientific Applications:

• DNA and RNA fluorescence in situ hybridization: Deconwolf enables accurate quantification of crowded diffraction-limited fluorescence dots in DNA and RNA FISH images.
• Tissue section transcript imaging: Deconwolf supports robust detection of individual transcripts in tissue sections imaged with x20 air objectives.
• In situ spatial transcriptomics: Deconwolf increases transcript identification more than threefold in deconvolved spatial transcriptomics images.
• Fluorescence in situ sequencing: Deconwolf improves chromosome tracing in images from fluorescence in situ sequencing of barcoded Oligopaint probes.

Methodology:

Deconwolf performs deconvolution of widefield fluorescence microscopy images.