dEMBF

dEMBF v2.0 provides a curated database of enzymes involved in microalgal lipid biosynthesis to support research into lipid metabolism and biofuel production.

Key Features:

• Expanded Enzyme Annotation: Contains 2018 sequences encoding 35 distinct enzymes, representing a greater-than-sevenfold expansion from the initial version.
• Increased Species Coverage: Covers 32 algal genomes to enable broader comparative analyses of lipid biosynthesis.
• Enhanced Metabolic Pathway Analysis: Provides analyses of additional metabolic pathways relevant to lipid accumulation in microalgae.
• Detailed Annotation Features: Includes sequence and structural annotations, explicitly reporting enzyme–ligand interactions.
• Integration of Experimental Evidence: Integrates supporting experimental evidence that links specific enzymes to enhanced lipid content in microalgae.

Scientific Applications:

• Biofuel Production Optimization: Facilitates identification of enzyme targets for genetic and metabolic engineering to increase lipid yields in microalgae.
• Comparative Genomics: Enables comparative studies across 32 algal genomes to investigate variation in lipid biosynthesis pathways.
• Functional and Structural Analysis: Supports functional studies and enzyme–ligand interaction analyses to inform selection of targets for manipulation.

Methodology:

Annotation of 2018 enzyme sequences, metabolic pathway analysis across 32 algal genomes, structural annotation including enzyme–ligand interactions, and integration of supporting experimental evidence.