DIALib

DIALib generates ion libraries for Data Independent Acquisition (DIA) mass spectrometry to enable identification and measurement of peptides and glycopeptides for comprehensive proteomic and glycoproteomic analysis.

Key Features:

• Automated ion library generation: Automates generation of ion libraries specifically for Data Independent Acquisition (DIA) mass spectrometry to support unbiased measurement of detectable peptides.
• Peptide and glycopeptide support: Targets both peptides and glycopeptides, including N- and O-glycopeptides.
• Theoretical and empirical libraries: Constructs both theoretical and empirically-derived ion libraries, enabling analysis when Data Dependent Acquisition (DDA)-derived libraries are limited.
• No prior glycan knowledge required: The theoretical approach enables analysis of diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of glycan structures.
• Oxonium-only libraries (oxoniome): Generates libraries consisting solely of glycan oxonium ions to provide compositional glycosylation profiles, termed the "oxoniome".
• Complementary to DDA: Complements DDA-derived ion libraries to broaden the scope of DIA glycoproteomics.

Scientific Applications:

• Compositional glycosylation profiling: Global profiling of glycosylation composition (oxoniome) across detectable glycoproteomes using oxonium ions.
• Yeast glycoproteome analysis: Identification and measurement of diverse glycopeptides in moderately complex yeast cell wall glycoproteomes.
• Mammalian glycoprotein mixtures: Analysis and measurement of glycopeptides in mixtures of mammalian glycoproteins.
• Enhanced DIA glycoproteomics: Enables DIA-based analysis of glycopeptides with complex post-translational modifications, complementing traditional DDA workflows.

Methodology:

Generates theoretical and empirically-derived ion libraries, including libraries composed solely of glycan oxonium ions for oxoniome profiling, to support Data Independent Acquisition (DIA) mass spectrometry analysis.