DIANA miRPath

DIANA-miRPath v2.0 performs pathway analysis to identify molecular pathways altered by single or multiple microRNAs using DIANA-microT-CDS predictions and TarBase v6 experimental targets.

Key Features:

• High-Accuracy miRNA Target Prediction: Uses the DIANA-microT-CDS algorithm and incorporates experimentally verified targets from TarBase v6 to identify miRNA targets.
• Integration and Meta-analysis: Merges results using advanced meta-analysis algorithms and performs hierarchical clustering of miRNAs and pathways based on interaction levels.
• Visualization: Produces dendrograms and heat maps depicting miRNA versus pathway interactions for interpretation of interaction patterns.
• Pathogenic SNPs Module: Provides information on pathogenic single nucleotide polymorphisms located within miRNA target sites.
• Reverse Search Module: Annotates predicted and experimentally validated miRNA targets within selected molecular pathways.

Scientific Applications:

• Gene Regulation Studies: Enables identification of pathways regulated by individual or combinations of miRNAs.
• Disease Mechanism Elucidation: Supports analysis of miRNA-mediated pathway alterations relevant to disease processes.
• Therapeutic Target Identification: Aids in pinpointing pathway components influenced by miRNAs that may serve as therapeutic targets.

Methodology:

Combines DIANA-microT-CDS predictions with TarBase v6 experimental targets, merges results via meta-analysis algorithms, applies hierarchical clustering, generates dendrograms and heat maps, and incorporates a SNPs module and reverse search annotation.