DICHROWEB

DICHROWEB analyzes circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD) spectra to predict protein secondary structure content.

Key Features:

• Algorithmic versatility: Supports multiple analysis algorithms including a choice among five established methods for CD spectra deconvolution and secondary structure estimation.
• Reference database integration: Integrates seven different reference databases for comparison and calibration of calculated secondary structure content.
• Supported spectral data sources: Accepts spectral data from commercial CD instruments, synchrotron radiation (SRCD) beamlines, and various spectral processing software formats.
• Output types: Produces calculated secondary structures, goodness-of-fit parameters, and tabular and graphical displays of experimental, calculated, and difference spectra.
• Graphical analysis: Provides graphical comparisons between calculated and experimental spectra to assess agreement and visualize residuals.

Scientific Applications:

• Protein secondary structure estimation: Derives quantitative secondary structure content (e.g., helix, sheet, coil) from CD and SRCD spectra.
• Structural biology and biochemical research: Supports analyses in structural biology, biochemistry, and molecular biology that require validation or characterization of protein secondary structure.

Methodology:

Integrates multiple algorithms (five selectable methods) with seven reference databases to analyze input CD/SRCD spectra in various formats, processes spectra with the selected algorithm(s) to predict secondary structure, and computes a goodness-of-fit parameter with tabular and graphical output of experimental, calculated, and difference spectra.