Digenome-seq web tool

A web tool for in vitro identification of potential nuclease (e.g. CRISPR-Cas method) off-target sites from whole-genome sequencing data.

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Topics

Whole genome sequencing Sequence analysis Molecular biology

Details

Maturity:: Mature
Cost:: Free of charge
Tool Type:: web application
Operating Systems:: Linux, Windows, Mac
Added:: 6/5/2018
Last Updated:: 11/25/2024

Operations

Nucleic acid feature detection
Sequence alignment
Validation

Data Inputs & Outputs

Nucleic acid feature detection

Inputs

RNA sequence (raw)
- Textual format

Outputs

Report
- TSV

Publications

Park J, Childs L, Kim D, Hwang G, Kim S, Kim S, Kim J, Bae S. Digenome-seq web tool for profiling CRISPR specificity. Nature Methods. 2017;14(6):548-549. doi:10.1038/nmeth.4262. PMID:28557982.

DOI: 10.1038/nmeth.4262

PMID: 28557982

Documentation

General

http://www.rgenome.net/digenome-js/help

Downloads

Binaries
http://www.rgenome.net/digenome-js/standalone

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