digilogger

digilogger analyzes fluorescence data from point-of-care testing (POCT) microfluidic microbead chip experiments to detect and quantify DNA and protein biomarkers for cardiovascular and outbreak-related monitoring.

Key Features:

• Simultaneous Detection and Quantification: Supports concurrent assessment of multiple biomarkers, including cell-free mitochondrial DNA (cfmDNA) and proteins such as C-reactive protein (CRP), brain natriuretic peptide (BNP), and low-density lipoprotein (LDL).
• Microfluidic Technology Integration: Operates with a microfluidic platform that uses 11–15 μm, dual-color encoded microbeads to immobilize biomarkers as planar layers within a flow cell for fluorescence measurements.
• Automated Fluorescence Analysis: Compatible with an in-house fully automated fluorescence microscope for end-point and real-time analysis of binding events between target molecules and microbeads, with reported optimization reducing detection limits by at least fivefold per biomarker.
• Rapid Data Processing: Enables rapid kinetic data inspection, reaching nearly 50% of fluorescence intensity within 7 minutes during kinetic experiments to support timely quantitative assessment.

Scientific Applications:

• Cardiovascular Disease Monitoring: Supports detection and quantification of biomarkers relevant to cardiovascular disease for diagnosis and monitoring studies.
• Disease Outbreak Detection: Facilitates rapid multi-biomarker assessment useful for identifying and tracking disease outbreaks.

Methodology:

Processes fluorescence microscopy end-point images and time-series from microbead-based microfluidic assays to provide visualization, kinetic analysis, and quantitative interpretation of binding events.