DISMISS

DISMISS detects strand-associated DNA methylation within Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) datasets to resolve strand-specific methylation signals.

Key Features:

• Strand-Specific Detection: Identifies symmetric and asymmetric DNA methylation patterns across the plus and minus genomic strands from MeDIP-Seq data.
• High Accuracy: Demonstrates an ~80% concordance rate with bisulfite sequencing (BS-Seq) data derived from Apis mellifera worker larvae.
• Precision at Splice Sites: Detects the strand origin of DNA methylation at splice site junctions with statistical significance (p < 0.0001) comparable to BS-Seq precision.
• Comprehensive Genomic Coverage: Identifies methylation signals across upstream, exonic, intronic, and downstream regions and reports improved detection relative to MACS2.

Scientific Applications:

• Analysis of low-methylation eukaryotic genomes: Enables detection of strand-specific methylation patterns in species or tissues with low overall DNA methylation levels.
• Study of asymmetric methylation: Facilitates investigation of genomes exhibiting high amounts of asymmetric methylation, including analyses in invertebrates such as Apis mellifera.

Methodology:

Processes existing MeDIP-Seq datasets to detect and differentiate symmetric (CG) and asymmetric methylation, identifies strand origin at splice junctions, and compares results to BS-Seq for validation.