dms-view

dms-view visualizes deep mutational scanning (DMS) data mapped onto three-dimensional protein structures to interpret mutation-level effects on protein function and stability.

Key Features:

• Integration with 3-D Protein Structures: Links site-specific and mutation-level DMS data directly to three-dimensional protein structures to provide spatial context for mutations.
• Site- and Mutation-level Data Exploration: Enables selection and inspection of individual sites and mutation-level measurements to examine detailed DMS effects.
• Flexible Input Handling: Accepts flexible input data files and supports user-specified protein structures plus additional data such as amino acid frequencies from natural alignments.
• Implementation Technologies: Uses JavaScript and D3.js for mapping and rendering DMS data onto structural representations.

Scientific Applications:

• Protein Engineering: Visualizes how specific amino acid substitutions affect protein structure and function to inform engineering decisions.
• Virology and Immunology: Maps mutational effects on viral proteins to study antigenic variation and immune escape.
• Clinical Research: Assists interpretation of human genetic variation by contextualizing mutation effects observed in patient populations.

Methodology:

Maps site-specific and mutation-level DMS measurements onto three-dimensional protein structures and renders the resulting visualizations using JavaScript and D3.js.