DNAmoreDB

DNAmoreDB catalogs deoxyribozymes (DNAzymes) by aggregating sequences, selection conditions including in vitro selection, reaction types, kinetic parameters, substrates, cofactors, structural data, and literature references to support DNAzyme research.

Key Features:

• Data collection and organization: Systematic compilation of DNAzyme sequences with associated selection conditions, kinetic parameters, substrates, cofactors, structural data, and literature citations.
• Catalytic diversity: Coverage of DNAzymes reported to catalyze 20 distinct chemical reactions acting on substrates such as DNA, RNA, peptides, and small organic compounds.
• Search capabilities: Support for keyword searches and BLASTN nucleotide queries to locate specific DNAzyme sequences and related metadata.
• Programmatic access: REST-based API providing machine-readable retrieval of database entries.

Scientific Applications:

• Basic research: Facilitating analysis of DNAzyme sequence–function relationships and investigation of catalytic mechanisms.
• Biotechnological development: Enabling discovery and comparison of DNAzyme candidates for biocatalysis, diagnostics, or therapeutic applications.
• Synthetic biology: Supporting design and integration of DNAzyme-based catalytic elements into engineered biological systems.

Methodology:

Systematic compilation and curation of literature-derived sequence and metadata; BLASTN for sequence queries; REST-based API for programmatic data access.