DoChaP

DoChaP visualizes exon-domain associations to reveal how alternative splicing alters protein domain composition across transcripts and between species.

Key Features:

• Exon-domain visualization: Visualizes exon-domain associations across all transcripts of a gene, mapping exons to protein domains.
• Protein-domain mapping: Displays encoded protein isoforms and their annotated domains to enable direct comparison between transcripts and protein products.
• Color-coded comparative visualization: Uses color-coded, organized displays to highlight structural changes in protein architecture resulting from alternative splicing.
• Two-species comparison: Performs pairwise exon-domain comparisons between two species to assess conservation of exons, splicing events, and domain architecture.
• Automated analysis across genes: Automates integration of exon and domain data to support multi-gene and scalable analyses that reduce manual integration efforts.

Scientific Applications:

• Alternative splicing impact analysis: Assess how alternative splicing modifies protein domain composition and isoform architecture.
• Evolutionary conservation studies: Investigate conservation of exons, splicing patterns, and domain arrangements between species.
• Structural consequence evaluation: Examine structural changes in protein architecture caused by splicing variants relevant to health and disease.
• Genomic-scale splicing studies: Support genomic investigations linking alternative splicing events to changes in protein domain organization.

Methodology:

Visualizes exon-domain associations across transcripts, displays encoded proteins with annotated domains, and performs comparative exon-domain analysis between two species.