dotpath (EBI)

dotpath (EBI) generates non-overlapping wordmatch dotplots of two sequences to visualize matching regions for comparative sequence analysis.

Key Features:

• Non-overlapping wordmatch dotplot generation: Creates dotplots that represent exact word matches between two sequences without overlapping matches to highlight matching regions.
• Integration with EMBL-EBI resources: Links to UniProt, InterPro, ENA, and Ensembl Genomes for use of reference sequences and annotation data.
• Scalability and large dataset handling: Updated to accommodate increasing data demands and to handle large datasets.

Scientific Applications:

• Comparative genomics: Visualizes sequence similarity to identify conserved and divergent regions between sequences.
• Evolutionary studies: Reveals matching regions between sequences to support comparative and evolutionary analyses.
• Functional annotation: Assists functional annotation by indicating sequence similarities that inform hypothesis generation.

Methodology:

The method aligns two sequences to identify non-overlapping matching regions which are represented as a wordmatch dotplot.