DrosOmics

DrosOmics enables exploration and analysis of genomic variation across high-quality de novo reference genomes from natural populations of Drosophila melanogaster, with emphasis on transposable-element-driven structural variation.

Key Features:

• Long-read sequencing support: Leverages long-read sequencing technologies to obtain multiple de novo genome assemblies.
• Reference genome collection: Hosts 52 high-quality reference genomes from natural populations of D. melanogaster represented as de novo assemblies.
• Transposable element annotations: Provides annotations from a curated, high-confidence set of transposable elements, the predominant class of structural variants in these genomes.
• Functional multi-omics integration: Integrates transcriptomics and epigenomics datasets for 26 genomes to link genetic variation with functional signals.
• Comparative visualization: Supports simultaneous visualization and comparison of multiple genome assemblies using the JBrowse 2 platform.
• Structural variant focus: Enables analysis of structural and functional genomic features across populations with particular attention to structural variants.

Scientific Applications:

• Population genomics: Characterizing genetic diversity and population-level variation in D. melanogaster using multiple de novo assemblies.
• Structural variant discovery: Identifying and characterizing transposable-element insertions and other structural variants across genomes.
• Functional genomics: Correlating structural variants with transcriptomic and epigenomic signals in 26 genomes.
• Evolutionary genetics: Investigating genetic architecture and evolutionary dynamics underlying phenotypic diversity and adaptation in natural populations.

Methodology:

Built on the JBrowse 2 platform, DrosOmics uses long-read sequencing-based de novo genome assemblies, curated transposable element annotations, integrated transcriptomics and epigenomics datasets for 26 genomes, and simultaneous visualization of multiple assemblies.