DSBS

DSBS decodes genome-wide DNA methylation and single-nucleotide variants from bisulfite sequencing data to enable simultaneous analysis of the DNA methylome and genomic variation.

Key Features:

• Simultaneous identification: Identifies genome-wide SNVs and DNA methylation at single-base resolution from a single DSBS dataset using a hairpin adapter to link Watson and Crick strands followed by bisulfite conversion and paired-end sequencing.
• Strand-bias elimination: Sequencing of both bisulfite-converted Watson and Crick strands within a single paired-end read removes strand bias in methylation and variant calls.
• Error correction: Mutual correction between read1 and read2 enables estimation and reduction of amplification and sequencing errors.
• Comprehensive analysis pipeline: DSBS Analyzer processes DSBS data to accurately call SNVs and quantify DNA methylation levels genome-wide.

Scientific Applications:

• Genome-wide hemimethylation landscape: Enables comprehensive profiling of hemimethylation distribution across genomic regions in human cells, including promoters and CpG islands.
• Disease and biological process studies: Facilitates combined analysis of genetic and epigenetic variation for research into diseases and biological processes influenced by both SNVs and DNA methylation.

Methodology:

DSBS Analyzer computationally processes DSBS paired-end reads to call SNVs and quantify methylation; mutual correction between read1 and read2 is used to estimate and reduce amplification and sequencing errors; strand-bias elimination is achieved by leveraging paired-end sequencing of both bisulfite-converted Watson and Crick strands.