DualSeqDB

DualSeqDB aggregates and standardizes dual RNA sequencing (dual RNA-seq) data to provide differential gene expression profiles of pathogenic bacteria and their hosts for mechanistic studies of host-pathogen interactions.

Key Features:

• Extensive Data Repository: Contains nearly 300,000 entries compiled from eight distinct studies representing differential gene expression in both bacteria and host organisms under in vivo and in vitro conditions.
• Standardized Analysis Pipeline: Analyzes raw transcriptomic data through a standardized pipeline to enable consistent comparisons across datasets.
• Diverse Organism Coverage: Includes data from seven strains of pathogenic bacteria and multiple cell types and tissues from Homo sapiens (human), Mus musculus (mouse), and Macaca fascicularis (rhesus macaque).
• Temporal Data Points: Provides expression measurements across multiple time points to capture infection dynamics.

Scientific Applications:

• Elucidation of Pathogenesis Mechanisms: Identification of transcriptional changes during infection to reveal bacterial virulence strategies and host responses.
• Development of Antimicrobials: Highlighting bacterial genes critical during infection to prioritize targets for antimicrobial development.
• Tailoring Therapeutic Interventions: Informing timing and targets for therapeutic interventions by linking host and pathogen expression patterns.

Methodology:

Raw high-throughput sequencing dual RNA-seq data are processed and analyzed through a standardized transcriptomic pipeline to derive differential gene expression entries.