easyFRAP-web

easyFRAP-web analyzes Fluorescence Recovery After Photobleaching (FRAP) datasets to quantify protein mobility and extract kinetic parameters such as mobile fraction and recovery half-time.

Key Features:

• Sequential analysis pipeline: Performs experimental data assessment, normalization, and estimation of curve-derived quantitative parameters in ordered processing steps.
• Data normalization: Normalizes fluorescence recovery curves to enable comparative analysis across experiments and cells.
• Curve fitting and quantitative parameter extraction: Applies curve fitting to derive kinetic metrics, including mobile fraction and recovery half-time.
• Batch evaluation of large datasets: Supports processing and evaluation of large numbers of FRAP curves for population-level analysis.
• Visualization of recovery curves: Provides visualization of normalized fluorescence recovery curves at each analysis step.
• Data export: Exports processed numerical data and figures generated during analysis for downstream analysis or reporting.

Scientific Applications:

• Protein dynamics studies: Quantifies mobility and kinetic behavior of proteins within living cells using FRAP time-series data.
• Single-cell heterogeneity analysis: Enables comparison of kinetic parameters across individual cells to investigate cell-to-cell variability.
• Comparative condition analysis: Facilitates comparison of protein dynamics between experimental conditions, such as presence or absence of perturbations.

Methodology:

Analysis of FRAP time-series including data assessment, normalization of fluorescence recovery curves, and curve fitting applied to datasets examining the mobility of the cell cycle regulator Cdt2 in cultured cells under conditions with and without DNA damage.