EasyDiver+

EasyDiver+ analyzes high-throughput sequencing data from in vitro evolution of nucleic acids or amino acids by processing raw paired-end demultiplexed Illumina read files, calculating enrichment values, and generating graphical representations of sequence metrics.

Key Features:

• Raw data processing: Processes raw, paired-end, demultiplexed Illumina read files.
• Enrichment analysis: Calculates enrichment values for each unique sequence across consecutive selection rounds.
• Visualization platform: Provides a highly flexible and customizable platform for detailed graphical representations of sequence metrics.

Scientific Applications:

• In vitro evolution of nucleic acids: Supports analysis of high-throughput sequencing data from in vitro evolution experiments identifying functional nucleic acids.
• In vitro evolution of amino acids: Supports analysis of high-throughput sequencing data from in vitro evolution experiments identifying functional peptides and proteins.

Methodology:

The tool builds on the foundation of the original EasyDIVER tool and introduces advanced analytical capabilities including enrichment value calculation and customizable visualization of sequence metrics.