Easymap

Easymap performs mapping-by-sequencing analyses to identify causal point mutations and large insertions from next-generation sequencing (DNA-seq or RNA-seq) data in mutant mapping populations.

Key Features:

• Comprehensive mapping capabilities: Supports bulked segregant mapping of EMS-induced point mutations and tagged-sequence mapping for large insertions such as transposons or T-DNAs using DNA-seq or RNA-seq datasets.
• Detailed reporting: Generates mapping reports that include images and complementary data to aid assessment of mapping results.
• Validation across datasets and species: Algorithms have been validated using experimental and simulated datasets across various plant and animal model species.

Scientific Applications:

• Genetic screens: Identification of causal mutations from mutants isolated in genetic screens.
• Mutation discovery: Detection and localization of point mutations and large insertions (e.g., transposons, T-DNAs) underlying phenotypic variants.
• Plant and animal genomics: Application to model species for studies of genetic contributions to traits and diseases.

Methodology:

Uses next-generation sequencing (NGS) data from mapping populations combined with classical linkage analysis to pinpoint causal mutations, with algorithms validated on experimental and simulated datasets.