EbolaID

EbolaID provides a quality-checked, regularly updated database of oligonucleotides and sequence-conservation measures for the Ebola virus to support design of nucleic acid-based diagnostics and therapeutics.

Key Features:

• Genetic diversity capture: Captures genetic diversity across the Ebola virus genome to inform selection of assay targets.
• Sequence conservation measures: Computes sequence conservation metrics for each target region derived from multiple sequence alignments.
• Oligonucleotide repository: Maintains a quality-checked, regularly updated list of oligonucleotides for PCR/qPCR primers and probes, siRNAs, and related nucleic acid reagents.

Scientific Applications:

• PCR/qPCR assay design: Supports selection of primers and probes for PCR and quantitative PCR (qPCR) assays that account for Ebola virus sequence variation.
• Therapeutic oligonucleotide design: Facilitates identification of conserved target regions for siRNAs and other therapeutic nucleic acid reagents.
• Assay robustness assessment: Enables evaluation of the risk of false-negative results due to sequence variation at primer, probe, or therapeutic binding sites in Ebola virus (Filoviridae).

Methodology:

Derives conservation metrics from multiple sequence alignments and compiles a quality-checked, regularly updated list of oligonucleotides.