eCALIBRATOR

eCALIBRATOR enables comparative analysis of functional genomics datasets to identify genes and biological pathways involved in biotic stress responses in Eucalyptus, with emphasis on Eucalyptus grandis.

Key Features:

• Comparative Analysis: Compares multiple transcriptomic datasets to identify common differentially expressed genes across biotic stress interactions.
• Supported Biotic Stressors: Includes datasets from interactions with Leptocybe invasa, Phytophthora cinnamomi, and Chrysoporthe austroafricana.
• Visual Outputs: Produces Venn diagrams and supports clustering and extraction of overlapping gene expression data among studies.
• Gene Ontology Enrichment Analysis: Performs GO enrichment to identify significant biological processes, including the term "response to organonitrogen compound."
• Identification of Key Genes: Identified 708 common differentially expressed Eucalyptus grandis genes and highlighted NITRATE TRANSPORTER 2.5 (NRT2.5) as differentially regulated in susceptible versus resistant interactions.
• Data Integration: Integrates various functional genomics datasets to enable cross-study comparative analyses.

Scientific Applications:

• Discovery of conserved defense genes: Enables identification of genes and pathways commonly regulated across multiple biotic stressors in Eucalyptus.
• Candidate gene prioritization: Supports prioritization of genes for functional characterization and resistance studies, exemplified by NRT2.5.

Methodology:

Integrates functional genomics datasets and performs comparative analyses using Venn diagram generation, clustering and extraction of gene sets, and gene ontology enrichment via visual and statistical tools.