EcoCyc

EcoCyc provides a curated database of genetic, metabolic, and regulatory information for Escherichia coli K-12 MG1655 to support analysis of gene functions, operons, protein complexes, metabolic pathways, and regulatory networks.

Key Features:

• Manual literature curation: Captures and organizes information derived from extensive literature review concerning individual gene products of Escherichia coli K-12 MG1655.
• Gene product functions: Records molecular functions and biological roles for individual proteins and RNAs.
• Regulatory annotations: Documents transcriptional, post-transcriptional, and protein-level regulatory inputs affecting gene expression and protein activity.
• Operons, complexes, and metabolic pathways: Describes organization of genes into operons, protein complexes, and curated metabolic pathways.
• Regulation Summary Diagram: Provides a graphical overview of all known regulatory inputs affecting genes and proteins.
• Signal transduction pathways: Supplies curated graphical representations of signal transduction pathways.
• Cellular and regulatory overviews: Summarizes cellular processes and regulatory networks for E. coli K-12 MG1655.

Scientific Applications:

• Gene function annotation: Support interpretation of experimental data and hypothesis generation for gene and protein function studies in Escherichia coli K-12 MG1655.
• Regulatory network analysis: Enable visualization and analysis of transcriptional, post-transcriptional, and protein-level regulatory interactions.
• Metabolic pathway analysis: Facilitate analysis of curated metabolic pathways and pathway-level insights into cellular metabolism.
• Operon and complex organization studies: Enable investigation of gene organization into operons and composition of protein complexes.
• Signal transduction analysis: Provide curated diagrams to analyze signaling pathways and their components.

Methodology:

Data are compiled by manual curation from extensive literature review of experimental studies on Escherichia coli K-12 MG1655.