ENACT

ENACT integrates advanced cell segmentation with Visium HD transcriptomics data to infer cell types across whole tissue sections.

Key Features:

• Cell Segmentation Integration: Integrates advanced cell segmentation with Visium HD transcriptomics data.
• Bin-to-Cell Assignment: Incorporates novel bin-to-cell assignment methods that enhance the accuracy of single-cell transcript estimates.
• Scalability: Scales to samples with hundreds of thousands of cells.
• Validation: Validated on diverse synthetic and real datasets.

Scientific Applications:

• Spatially Resolved Transcriptomics Analysis: Provides a robust solution for spatially resolved transcriptomics analysis at cell resolution.
• Cell Type Inference: Infers cell types across whole tissue sections from Visium HD data.

Methodology:

ENACT is a self-contained pipeline that integrates advanced cell segmentation with Visium HD transcriptomics data and incorporates novel bin-to-cell assignment methods to enhance single-cell transcript estimate accuracy.