EnhFFL

EnhFFL catalogs enhancer-mediated feed-forward loops (FFLs) in human and mouse genomes to support analysis of transcriptional regulatory motifs and their roles in biological processes and diseases, including tumorigenesis.

Key Features:

• Extensive Data Coverage: Contains 46,280/7,000 TF-enhancer-miRNA FFLs, 9,997/236 enhancer-miRNA-gene FFLs, 3,561,164/3,193,182 TF-enhancer-gene FFLs, and 1,259/235 TF-enhancer feed-back loops (FBLs) across 91 tissues or cell lines for human and mouse.
• Element-level Annotations: Provides annotations and queryable attributes for elements including name/ID, genomic location, and conservation status of miRNA target genes.
• Customized Screening: Enables screening of customized FFL sets using thresholds such as q-value and confidence scores for miRNA target genes.
• Disease and Functional Enrichment: Includes disease-association and functional enrichment analyses that highlight master miRNAs implicated in tumorigenesis via TF-enhancer-miRNA and enhancer-miRNA-gene networks.

Scientific Applications:

• Gene Regulation Analysis: Supports investigation of enhancer-mediated FFLs as transcriptional network motifs in human and mouse.
• miRNA Regulatory Studies: Enables analysis of miRNA involvement in FFLs, including target confidence and conservation characteristics.
• Disease Mechanism and Cancer Research: Facilitates exploration of disease associations and the role of master miRNAs in tumorigenesis within FFL contexts.

Methodology:

Compilation of extensive FFL datasets across multiple tissues and cell lines and integration of tools for customized screening and enrichment analysis.