enset

enset catalogs microsatellite (simple sequence repeat, SSR) markers for Ensete ventricosum to support genetic diversity assessment, genotyping, and breeding-related genomic studies.

Key Features:

• Microsatellite identification: 233 microsatellites (SSRs) per megabase pair (Mbp) were identified in the Ensete ventricosum genome, representing approximately 0.28% of the genomic content.
• Marker development: 154,586 non-redundant microsatellite markers were identified and 40 markers were selected for primer development.
• Polymorphism and genetic diversity: 92.5% of developed markers were polymorphic with a Polymorphism Information Content (PIC) of 0.87 and expected heterozygosity (He) ranging from 0.79 to 0.82.
• Cross-species transferability: In silico analysis showed 46.86% marker transferability among enset species and 1.90% transferability to Musa (banana).
• Database (EnMom@base): EnMom@base centralizes the identified SSR markers and associated marker information for Ensete ventricosum.
• Data deposition: SSR and marker datasets were deposited to Figshare.

Scientific Applications:

• Genetic diversity assessment: SSR markers enable measurement of genetic diversity and characterization of Ensete ventricosum landraces.
• Genotyping and selection: Markers support genotyping and selection workflows relevant to breeding and marker-assisted selection.
• Comparative genomics: Transferability to other enset species and Musa facilitates comparative genomic analyses within the Musaceae.
• Conservation and germplasm management: Molecular marker information supports conservation strategies and germplasm characterization.

Methodology:

Microsatellite (SSR) identification from the Ensete ventricosum genome, in silico transferability analysis, and selection of 40 markers for primer development.