EntropyExplorer

EntropyExplorer analyzes gene expression data using Differential Shannon Entropy (DSE) and Differential Coefficient of Variation (DCV) to quantify variability differences across conditions.

Key Features:

• Calculation of DSE and DCV: Computes Differential Shannon Entropy (DSE) and Differential Coefficient of Variation (DCV) to quantify entropy and coefficient-of-variation differences in gene expression across conditions.
• Automated p-value computation: Computes p-values for DSE, DCV, and differential expression (DE) metrics.
• Statistical test for DCV: Uses the Fligner-Killeen test to compute p-values for DCV.
• P-value computation for DSE: Uses permutation testing to compute p-values for DSE.
• Variability-focused analysis: Provides variability-focused analyses that complement magnitude-focused differential expression methods.

Scientific Applications:

• Gene expression variability analysis: Quantifies changes in expression variability to identify genes with altered stability or consistency across biological conditions.
• Complementary analysis to differential expression: Complements magnitude-focused DE analyses by highlighting genes with significant changes in entropy or coefficient of variation across conditions.

Methodology:

Computes DSE and DCV metrics; computes p-values for DCV using the Fligner-Killeen test; computes p-values for DSE using permutation testing; computes p-values for DE.