EPIphany

EPIphany analyzes and visualizes peptide immunoarray data to characterize antibody responses and identify antigenic determinants (putative linear epitopes).

Key Features:

• High-density peptide microarray data support: Handles data derived from high-density peptide microarrays that represent diverse populations of putative linear epitopes.
• Data normalization options: Provides various data normalization techniques to mitigate experimental variability in immunoarray measurements.
• Data analysis parameters: Exposes tunable analysis parameters to customize processing and statistical evaluation of immunoarray datasets.
• Diverse visualization tools: Generates multiple visualization outputs to display patterns of antibody reactivity and comparative results across datasets.

Scientific Applications:

• Antigenic determinant mapping: Identifies specific linear antigenic determinants by analyzing antibody binding to peptide arrays.
• Vaccine development and assessment: Assesses breadth and specificity of humoral responses relevant to rational vaccine design and evaluation.
• High-throughput antibody population profiling: Enables large-scale examination of antibody repertoires using peptide immunoarray measurements.

Methodology:

Processes immunoarray datasets derived from high-density peptide microarrays representing putative linear epitopes using data normalization, parameterized analysis, and visualization of antibody reactivity.