Escher-Trace

Escher-Trace analyzes and visualizes stable isotope tracing mass spectrometry (MS) data to map metabolite labeling, including ^13C experiments, onto annotated metabolic pathways.

Key Features:

• Baseline-corrected MS input: Accepts baseline-corrected mass spectrometry (MS) tracing data as input for downstream analysis.
• Natural isotope abundance correction: Performs correction for natural isotope abundance on tracer-derived MS data.
• Metabolite labeling visualization: Generates high-quality graphs that depict metabolite labeling patterns from stable isotope experiments, including ^13C labeling.
• Pathway integration: Maps labeling data onto annotated metabolic pathways or user-provided custom networks to contextualize labeling patterns.

Scientific Applications:

• Metabolic pathway elucidation: Supports experiments that trace label incorporation to elucidate metabolic pathway activity.
• Metabolic flux investigation: Aids investigation of metabolic fluxes by quantifying and visualizing isotope labeling patterns.
• Metabolomics and biochemistry: Facilitates interpretation of stable isotope tracing data in metabolomics and biochemical studies.
• Systems biology and network dynamics: Enables exploration of labeling dynamics within biochemical networks under varied conditions.

Methodology:

Input baseline-corrected MS tracing data; perform natural isotope abundance correction; generate visualizations of metabolite labeling patterns; map results onto user-provided or annotated metabolic pathways.