ESO

ESO designs evolutionarily stable genetic constructs by analyzing sequence features to reduce mutational degradation while maintaining host expression levels.

Key Features:

• Automatic Design: Automates design of genetic constructs that resist evolutionary loss-of-function mutations.
• Hotspot Avoidance: Identifies and avoids mutational and epigenetic hotspots that are prone to destabilizing constructs.
• Custom Hotspot Definition: Allows definition of custom hotspots specific to particular applications or organisms.
• Balancing Stability with Expression: Considers guanine-cytosine (GC) content and codon usage of the host to optimize between evolutionary stability and high gene expression.

Scientific Applications:

• Synthetic Biology: Supports long-term functionality of engineered constructs for applications such as metabolic engineering and gene therapy.
• Genetic Research: Provides insights into evolutionary dynamics by enabling analysis and stabilization of sequences against natural selection pressures.

Methodology:

Employs a computational approach that analyzes sequence data for mutational tendencies, identifies mutational and epigenetic hotspots, considers host-specific factors such as GC content and codon usage, and produces optimized designs that maintain functionality without compromising expression levels.