EV-DNA

EV-DNA aggregates curated information on extracellular vesicle-associated DNA (EV-DNA) from clinical and in‑vitro human biofluid samples to support liquid biopsy and biomarker research.

Key Features:

• Curated Data Collection: Manually curated datasets are extracted from peer‑reviewed literature with validated experimental details.
• Source Types: Includes data derived from both clinical samples and in‑vitro studies.
• Searchable Parameters: Records include EV isolation methods, characterization techniques, DNA isolation protocols, quality validation processes, DNA fragment size, starting material volume, gene names, and disease context.
• Disease Representation: Contains samples from 23 distinct diseases.
• Technological Diversity: Documents 13 EV isolation techniques applied across eight human biofluids, including blood and saliva.
• Genomic Focus: Features whole genome data and targeted entries for oncogenes KRAS, EGFR, BRAF, MYC, and mitochondrial DNA (mtDNA).
• Compliance Scoring System: Assigns compliance scores based on MISEV guidelines derived from experimental parameters reported in each study.

Scientific Applications:

• Biomarker Discovery: Supports identification and comparison of EV‑DNA biomarkers across studies and disease contexts.
• Liquid Biopsy and Cancer Diagnostics: Facilitates evaluation of EV‑DNA for noninvasive cancer detection and monitoring.
• Personalized Medicine: Aids assessment of oncogene and mtDNA alterations for patient stratification and treatment considerations.
• Mechanistic Studies: Enables investigation of EV‑DNA roles in intercellular communication under normal and pathological conditions.
• Comparative Disease Analysis: Allows cross-disease analyses leveraging data from 23 represented diseases.

Methodology:

The database entries are manually curated from peer‑reviewed literature and the database is updated annually to incorporate newly published EV‑DNA data.