EvoACTG

EvoACTG provides a comparative repository of chamber-specific cardiac transcriptomes and homologous genes across seven vertebrate species to enable evolutionary analysis of protein-coding genes, long non-coding RNAs (lncRNAs), and circular RNAs (CircRNAs).

Key Features:

• Chamber-Specific Transcriptomes: Differentially expressed genes are cataloged for distinct heart chambers across seven vertebrate species, including protein-coding genes, lncRNAs, and CircRNAs.
• Evolutionary Conservation: Genes, lncRNAs, and pathways conserved across vertebrates are highlighted to reveal evolutionary aspects of cardiac function.
• LncRNA Homolog Identification: Homologous lncRNAs are identified using sequence similarity, secondary structure, synteny, and expressional conservation, with emphasis on syntenic conservation.
• Transcript Co-expression Factors: Factors influencing transcript co-expression are analyzed, including genomic overlap, strandedness, and transcript biotype.
• CircRNA Catalogue: A catalogue of abundantly expressed CircRNAs across vertebrate hearts is provided.

Scientific Applications:

• Molecular Mechanism Exploration: Enables investigation of transcriptional mechanisms underlying heart function and disease using chamber-specific differentially expressed transcripts.
• Therapeutic Target Discovery: Conserved expression patterns across species can inform identification of candidate targets relevant to cardiovascular disease.
• Comparative Genomics: Supports comparative genomics studies of protein-coding genes, lncRNAs, and CircRNAs across seven vertebrate species.

Methodology:

Analysis of cardiac transcriptomes from seven vertebrate species with identification and cataloging of differentially expressed genes, lncRNAs, and CircRNAs by heart chamber, evaluation of evolutionary conservation via sequence similarity, secondary structure, synteny, and expressional conservation, and analysis of co-expression factors such as genomic overlap, strandedness, and transcript biotype.