ExpressAnalyst

ExpressAnalyst facilitates processing, annotation, and ortholog-based functional analysis of RNA-seq data to derive gene-level and cross-species biological insights across diverse eukaryotic species.

Key Features:

• Modular Workflow: Integrated modules perform processing and annotation of FASTQ files, statistical analyses, and functional assessments of count tables or gene lists.
• EcoOmicsDB Integration: Uses the EcoOmicsDB ortholog database to enable ortholog-based analyses for species lacking reference transcriptomes.
• Ultra-Fast Read Mapping Algorithms: Employs ultra-fast read mapping algorithms to generate global expression profiles and gene-level results from raw reads within 24 hours.
• Non-model Species Support: Supports RNA-seq analysis across diverse eukaryotic organisms, including those without reference transcriptomes.

Scientific Applications:

• Non-model organism transcriptomics: Analysis of RNA-seq datasets from non-model eukaryotes to identify differential expression and functional signatures.
• Cross-species functional inference: Ortholog-based mapping via EcoOmicsDB to transfer annotation and infer gene function across species.
• Salamander comparative studies: Comparative expression analysis of multiple non-model salamander species, including those without reference transcriptomes.

Methodology:

Processing and annotation of FASTQ files, ultra-fast read mapping algorithms coupled with the EcoOmicsDB ortholog database, followed by statistical analyses and functional assessments of count tables or gene lists.