FAST MS

FAST MS identifies, assigns, and relatively quantifies signals of molecular and fragment ions in top-down mass spectra of biopolymers with known sequence, including RNA, DNA, and proteins, and provides a toolbox for statistical analysis.

Key Features:

• Signal Identification and Assignment: Searches mass spectra for ion signals by comparing all signals with isotopic profiles calculated from known sequences.
• Relative Quantification: Quantifies signals of molecular and fragment ions in MS and MS/MS spectra.
• Modification Analysis: Enables accurate identification, localization and relative quantification of modifications, even in complex mixtures of positional isomers of proteins, oligonucleotides, or any other user-defined linear polymer.
• Statistical Analysis Toolbox: Provides a toolbox for statistical analysis of mass spectrometry data.
• Superior Sensitivity: Achieves superior sensitivity and an increased number of assigned fragment ions compared to algorithms that rely on artificial monomer units while maintaining the false positive rate below 5%.

Scientific Applications:

• Modified Biopolymer Characterization: Comprehensive characterization of modified proteins and nucleic acids by top-down mass spectrometry.
• Binding Site Mapping: Mapping of binding sites for native or therapeutic ligands when native electrospray ionization is used.
• Positional Isomer Mixture Analysis: Analysis of complex mixtures of positional isomers of proteins, oligonucleotides, or other user-defined linear polymers.

Methodology:

FAST MS searches mass spectra for ion signals by comparing all signals in the spectrum with isotopic profiles calculated from known sequences.