FAT-PTM

FAT-PTM provides a database and analysis resource that aggregates eight types of post-translational modifications across over 49,000 identified sites to map PTMs onto the Arabidopsis proteome and more than 600 Plant Metabolic Network pathways for analysis of PTM distribution, comodification, and quantitative phosphorylation dynamics.

Key Features:

• Comprehensive PTM dataset: Aggregates publicly available proteomic analyses covering eight PTM types across over 49,000 identified sites.
• Protein Search Tool: Searches the Arabidopsis proteome to retrieve associated PTMs and report quantitative changes in phosphorylation sites across ten experimental conditions.
• Metabolic Pathway Analysis Tool: Maps PTMs to over 600 metabolic pathways compiled from the Plant Metabolic Network to contextualize modifications within plant metabolism.
• Comodification Tool: Identifies protein groups that carry two or more user-defined PTMs to enable analysis of PTM comodification and cross-talk.

Scientific Applications:

• PTM distribution and dynamics: Investigating the distribution and quantitative dynamics of PTMs across the Arabidopsis proteome.
• Pathway-centered PTM analysis: Assessing the presence and potential roles of PTMs within metabolic pathways using Plant Metabolic Network mapping.
• PTM cross-talk studies: Studying comodification patterns and interactions among multiple PTMs on the same proteins.
• Regulatory role investigation: Examining the regulatory roles of PTMs in eukaryotic systems, with emphasis on plant biology.

Methodology:

Aggregates publicly available proteomic data, links PTMs to the Arabidopsis proteome, integrates over 600 pathways from the Plant Metabolic Network, and identifies protein groups with two or more user-defined PTMs and quantitative phosphorylation changes across ten experimental conditions.