FBPP: Foodborne Pathogen Primer Probe Design

FBPP designs PCR primers and probes for nucleic acid-based detection of foodborne pathogens.

Key Features:

• Primer and Probe Design: Designs primers and probes for detection of foodborne pathogen virulence factors using an SQL database.
• PCR Simulation: Simulates PCR reactions to predict amplification outcomes.
• Gel Electrophoresis Simulation: Generates simulated gel electrophoresis images.
• Specificity Checking: Checks the specificity of designed primers and probes against target sequences.

Scientific Applications:

• Foodborne Pathogen Detection: Supports nucleic acid-based detection methods for identifying foodborne pathogens in food samples.
• Virulence Factor Targeting: Enables design of oligonucleotides targeting virulence factors stored in the SQL database.

Methodology:

FBPP is a Python-based application supported by an SQL database containing foodborne pathogen virulence factors.