FDS

FDS quantifies functional divergence between orthologous human and mouse genes with emphasis on microRNA regulation.

Key Features:

• Comprehensive evaluation: Assesses functional divergence by analyzing microRNAs regulating paired orthologous genes between human and mouse.
• Gene-specific FDS values: Calculates a gene-specific Functional Divergence Score that is associated with evolutionary characteristics and expression divergence.
• Drug target differentiation: Distinguishes between targets of approved drugs and targets that failed in clinical trials based on orthologous gene analysis.
• Detailed functional and phenotypic insight: Reports functional and phenotypic differences for each orthologous gene pair.
• Extensibility: Framework is adaptable to analyze orthologous genes between human and other species.

Scientific Applications:

• Translational research: Quantifies cross-species functional differences to inform selection and interpretation of animal models for human disease studies.
• Drug development: Identifies orthologous gene divergence that may underlie differential drug target behavior between human and mouse.
• Evolutionary and genomic studies: Enables investigation of how gene functions and regulatory relationships, including microRNA interactions, have diverged between species.

Methodology:

Integrates microRNA regulation and gene expression pattern data to compute a gene-specific divergence score (FDS) for orthologous human–mouse gene pairs, with the score reflecting functional and phenotypic differences and associations with evolutionary characteristics and expression divergence.