FEVER

FEVER facilitates evolutionary transcriptomic analyses across teleost fishes by integrating high-quality genomic sequences and comprehensive transcriptomic datasets to reconstruct gene trees and compare tissue-specific expression.

Key Features:

• Gene Tree Reconstruction: Reconstructs gene trees from query genes to elucidate orthologous and paralogous relationships among 13 representative fish species spanning major evolutionary lineages and four model species used as evolutionary outgroups.
• Unbiased Gene Expression Data: Provides unbiased expression data across 11 tissues using the latest genomic annotations for multiple fish species to enable tissue-specific expression comparisons.
• Integration of Genomic and Transcriptomic Data: Integrates genomic sequences and transcriptomic datasets to investigate gene expression evolution in relation to speciation and gene duplication events.

Scientific Applications:

• Comparative genomics and phylogenomics: Supports large-scale comparative genomics studies across diverse fish species to infer evolutionary relationships and gene family histories.
• Gene function conservation and divergence: Enables analysis of conserved and diverged gene functions across species and tissues.
• Adaptive evolution: Facilitates investigation of adaptive evolution and the molecular basis of phenotypic diversity among fishes.
• Expression evolution after speciation/duplication: Allows study of how gene expression patterns have evolved following speciation events or gene duplications.

Methodology:

Combines high-quality genomic sequences from recent sequencing efforts with transcriptomic datasets and employs bioinformatics techniques to reconstruct gene trees and analyze gene expression across multiple tissues and species.