FiberQ

FiberQ quantifies DNA fibers in fluorescence microscopy images to measure DNA replication kinetics by automated analysis of fiber length and color.

Key Features:

• Implementation: MATLAB-based script implementing the quantification algorithm.
• Automation: Fully automated algorithm for unbiased quantification of DNA fibers.
• Measurements: Quantifies both the length and the color of DNA fibers.
• Image processing techniques: Uses edge detection for fiber identification.
• Segment splicing: Implements a novel segment splicing approach to assemble fiber segments.
• Input data: Processes fluorescence microscopy and immunofluorescence microscopy images.
• Validation: Benchmarking against manual assessments confirmed reliability of quantifications.

Scientific Applications:

• DNA replication kinetics: Enables quantitative analysis of replication fork progression and replication dynamics from DNA fiber assays.
• DNA fiber fluorography analysis: Automates processing and quantification of immunofluorescence microscopy–based DNA fiber experiments.

Methodology:

Implemented as a MATLAB script that applies image processing, including edge detection and a novel segment splicing approach, to automatically identify and quantify DNA fiber length and color from fluorescence/immunofluorescence microscopy images.