FISHalyseR

FISHalyseR quantifies and maps Fluorescence In Situ Hybridization (FISH) probes in digital cell culture images to extract spatial nuclear information for spatial co-localisation analysis.

Key Features:

• Quantification of FISH Probes: Measures presence and intensity of FISH probes within nuclear regions to provide quantitative data for genomic studies.
• Spatial Location Extraction: Extracts precise spatial coordinates for each nucleus and associated FISH probe to enable detailed spatial mapping.
• Co-localisation Analysis: Determines spatial relationships between nuclei and probes to support in-depth spatial co-localisation studies.
• Bioconductor Integration: Integrates with Bioconductor (R) to leverage its statistical frameworks and continuous testing for downstream analysis.
• Advanced Image Processing Algorithms: Applies advanced image processing algorithms to analyze digital cell culture images and extract spatial information.

Scientific Applications:

• Gene Expression and Chromosomal Abnormalities: Provides quantitative probe measurements useful for analyzing gene expression and detecting chromosomal abnormalities.
• Spatial Genome Organization Studies: Enables investigation of spatial genome organization within cells through mapped probe and nuclear coordinates.
• Cytogenetic Analyses: Enhances precision of cytogenetic analyses by delivering precise quantification and localization of FISH probes.

Methodology:

Leverages advanced image processing algorithms to analyze digital cell culture images and integrates with Bioconductor (R) to apply its statistical frameworks and continuous testing.